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cell death discovery  (Proteintech)


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    Structured Review

    Proteintech cell death discovery
    Cell Death Discovery, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 2375 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+death+discovery/CEL+Antibody/pm40436859-229-12-45
    Average 96 stars, based on 2375 article reviews
    cell death discovery - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Mechanical stiffness promotes skin fibrosis through Piezo1-mediated arginine and proline metabolism.
    Article Snippet: After blocking with 5% BSA at room temperature (RT) for 1 h, the membranes were incubated with the following primary antibodies: α-SMA (#19245, 1:1000, Cell Signaling Technology, Danvers, MA), fibronectin (ab2413, 1:1000, Abcam, Cambridge, UK), arginase-1 (Arg1) (16001-1-AP, 1: 5000, Proteintech, Wuhan, China), ornithine δ-aminotransferase (OAT) (A6235, 1:1000, ABclonal Technology, Wuhan, China), PYCR1 (13108-1-AP, 1:4000, Proteintech), proline dehydrogenase (oxidase) 1 (PRODH) (22980-1- AP, 1:2000, Proteintech) and β-actin (20536-1-AP, 1:5000, Proteintech) at 4 °C overnight. .. The next day, the membranes were incubated with HRPconjugated goat anti-rabbit IgG(H+ L) (SA00001-2, 1:10000, Proteintech) Cell Death Discovery (2023) 9:354 for 1 h at room temperature and then washed three times for 10min with tris-buffered saline containing 0.1% Tween 20. ..

    Article Title: LASS2 enhances p53 protein stability and nuclear import to suppress liver cancer progression through interaction with MDM2/MDMX.
    Article Snippet: After the cells were fixed and permeabilized, the cells were blocked with 10% goat serum and incubated overnight at 4 °C with a primary antibody against Ki-67 (Proteintech, 273099-1-AP, 1:1000). .. The cells were incubated Cell Death Discovery (2023) 9:414 with CoraLite594-conjugated goat anti-rabbit IgG (Proteintech, SA0013-4, 1:100). .. For the final step, the cells were incubated with Hoechst 33342 (CST, 4082S, 1:1000) for 15min.

    Article Title: Peroxiredoxin 1 regulates crosstalk between pyroptosis and autophagy in oral squamous cell carcinoma leading to a potential pro-survival.
    Article Snippet: .. 0.83 Cell Death Discovery (2023) 9:425 anti-Prdx1 (1:1000, Abcam, ab15571), anti-MMP2 (1:1000, Merck, MAB3308), anti-MMP9 (1:1000, Abcam, ab283575), anti-caspase-1 (1:1000, Abcam, ab1872), anti-GSDMD (1:1000, Abcam, ab210070), anti-NLRP3 (1:500, Abcam, ab214185), anti-p62 (1:1000, Proteintech, 11306-1-AP), anti-beclin1 (1:1000, Abcam, ab302669), anti-p-mTOR (1:1000, Abcam, ab109268), anti-LC3B (1:1000, Abcam, ab48394) and then followed by the incubation with the secondary antibodies (anti-rabbit 1:2000, Abcam, ab6721; anti-mice 1:2000, CellSignaling, #7076) at room temperature for 1 h. Images were captured by the gel imaging system (Amersham Imager 600; General Electric Company) with the enhanced chemiluminescence (ECL) kit. ..

    Article Title: NOXA exacerbates endoplasmic-reticulum-stress-induced intervertebral disc degeneration by activating apoptosis and ECM degradation.
    Article Snippet: .. The first antibodies were incubated overnight at 4 °C with the following Cell Death Discovery (2025) 11:257 dilutions: NOXA (ab222852, 1:1000, Abcam, Cambridge, UK), PERK (bs2469R, 1:1000, Bioss, Beijing, China), BAX (bs-0127R, 1:1000, Bioss, Beijing, China), BCL-2 (bs-0032R, 1:2000, Bioss, Beijing, China), MCL-1 (16225-1-AP, 1:1000, Proteintech, Wuhan, China), MMP3 (17873-1-AP, 1:1000, Proteintech, Wuhan, China), MMP13 (18165-1-AP, 1:1000, Proteintech, Wuhan, China), Cleaved Caspase-3 (25128-1-AP, 1:1000, Proteintech, Wuhan, China), ACAN (bs-1223R, 1:1000, Bioss, Beijing, China), COL2 (bs-5881R, 1:1000, Bioss, Beijing, China), GAPDH (10494-1-AP, 1:5000, Proteintech, Wuhan, China). .. After washing with TBST, the membranes were incubated with a secondary antibody conjugated to HRP (Proteintech, Wuhan, China) for 1 h at room temperature.

    Saline:

    Article Title: Mechanical stiffness promotes skin fibrosis through Piezo1-mediated arginine and proline metabolism.
    Article Snippet: After blocking with 5% BSA at room temperature (RT) for 1 h, the membranes were incubated with the following primary antibodies: α-SMA (#19245, 1:1000, Cell Signaling Technology, Danvers, MA), fibronectin (ab2413, 1:1000, Abcam, Cambridge, UK), arginase-1 (Arg1) (16001-1-AP, 1: 5000, Proteintech, Wuhan, China), ornithine δ-aminotransferase (OAT) (A6235, 1:1000, ABclonal Technology, Wuhan, China), PYCR1 (13108-1-AP, 1:4000, Proteintech), proline dehydrogenase (oxidase) 1 (PRODH) (22980-1- AP, 1:2000, Proteintech) and β-actin (20536-1-AP, 1:5000, Proteintech) at 4 °C overnight. .. The next day, the membranes were incubated with HRPconjugated goat anti-rabbit IgG(H+ L) (SA00001-2, 1:10000, Proteintech) Cell Death Discovery (2023) 9:354 for 1 h at room temperature and then washed three times for 10min with tris-buffered saline containing 0.1% Tween 20. ..

    other:

    Article Title: Arginine methylation-dependent TRIM47 stability mediated by CARM1 promotes the metastasis of hepatocellular carcinoma.
    Article Snippet: Cell Death Discovery (2024) 10:477 10494-1-AP), anti-Ubiquitin (Proteintech, 10201-2-AP), anti-Myc (Abclonal, AE010), anti-FLAG (Abclonal, AE005), Asymmetric di-methyl arginine antibody (Cell Signaling Technology, 13522), anti-E-cadherin (Proteintech,20874-1-AP), anti-N-cadherin (Proteintech, 22018-1-AP), anti-RHOA (Proteintech, 10749-1-AP), anti-Rac1 (Proteintech, 24072-1-AP), anti-AXIN2 (Proteintech, 20540-1-AP), anti-β-Catenin (Proteintech, 51067-2-AP), antiGST (Proteintech,10000-0-AP), anti-CRBN (Proteintech, 28494-1-AP), antiSNAI1 (Proteintech, 13099-1-AP), anti-SNAI2 (Cell Signaling Technology, 9585), anti-Vimentin (Cell Signaling Technology, 5741), anti-ZEB1 (Cell Signaling Technology, 3396), anti-ZO-1(Cell Signaling Technology, 5406).

    Recombinant:

    Article Title: Targeting MDM2 affects spastin protein levels and functions: implications for HSP treatment.
    Article Snippet: .. Cell Death Discovery (2025) 11:53 Technologies) or spastin recombinant fragment containing the MIT domain (79–298 aa; Proteintech Group, Rosemont, IL, USA) in 50mM Tris-HCl, pH 7.5, 150 nM NaCl. ..

    Imaging:

    Article Title: Peroxiredoxin 1 regulates crosstalk between pyroptosis and autophagy in oral squamous cell carcinoma leading to a potential pro-survival.
    Article Snippet: .. 0.83 Cell Death Discovery (2023) 9:425 anti-Prdx1 (1:1000, Abcam, ab15571), anti-MMP2 (1:1000, Merck, MAB3308), anti-MMP9 (1:1000, Abcam, ab283575), anti-caspase-1 (1:1000, Abcam, ab1872), anti-GSDMD (1:1000, Abcam, ab210070), anti-NLRP3 (1:500, Abcam, ab214185), anti-p62 (1:1000, Proteintech, 11306-1-AP), anti-beclin1 (1:1000, Abcam, ab302669), anti-p-mTOR (1:1000, Abcam, ab109268), anti-LC3B (1:1000, Abcam, ab48394) and then followed by the incubation with the secondary antibodies (anti-rabbit 1:2000, Abcam, ab6721; anti-mice 1:2000, CellSignaling, #7076) at room temperature for 1 h. Images were captured by the gel imaging system (Amersham Imager 600; General Electric Company) with the enhanced chemiluminescence (ECL) kit. ..



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